DNA

Part:BBa_K1039025:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-11)


Bxb1 Invertible Strong Promoter Switch Test Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 749
    Illegal NheI site found at 949
    Illegal NheI site found at 972
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 1872
    Illegal AgeI site found at 1984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal BsaI site found at 781
    Illegal BsaI.rc site found at 1263


Design Notes

To have expression on the prefix side of the switch, an "inverted" message must be used. The promoter of the switch is facing the prefix side and promotes the ORF to be read from suffix to prefix. In this case, "inverted" means the ORF is read from suffix to prefix.


Source

The switches and iGFP were synthesized by BioBasic inc.

References